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Reverse Transcription and Polymerase Chain Reaction: Principles and Applications in Dentistry
You will receive 1 credit(s) of continuing education credit upon successful completion of this course.
Description:

The purpose of this work is to present the details of PCR and RT-PCR in an attempt to clarify the principles and applications of both techniques in dentistry. 

Author:
Carlos Ferreira dos Santos, et al., DDS, PhD

Carlos Ferreira dos Santos, DDS, PhD, Assistant Professor, Bauru School of Dentistry, University of São Paulo, Brazil 

Vivien Thiemy Sakai, DDS, Graduate Student, Bauru School of Dentistry, University of São Paulo, Brazil 

Maria Aparecida...

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Learning Objectives:

Upon completion of this course, the participant should be able to do the following: 

  1. Understand the principles and applications of PCR and RT-PCR techniques in dentistry.
  2. Identify the use of PCR and RT-PCR in recent dental research.
  3. Recognize the potential of PCR and RT-PCR techniques for diagnosis, treatment, and prevention of many dental diseases.
Abstract:

Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that can serve as templates for different kinds of analyses. PCR can be preceded by a reverse transcription (RT) reaction in order to produce cDNA from RNA (RT-PCR). RT-PCR provides the possibility to assess gene transcription in cells or tissues. PCR and RT-PCR techniques have been instrumental in dental research, and show potential to be used for diagnosis as well as for treatment and prevention of many diseases (dental caries, periodontal disease, endodontic infections and oral cancer). Compared to other traditional methodologies, PCR and RT-PCR show many advantages including high specificity, sensitivity, and speed. Since PCR and RT-PCR are relatively new techniques and are not available to most students and professionals involved with dentistry, the aim of this work is to present the details of these techniques as well as dental literature reports in which they were used.

Keywords: Dental caries; Periodontitis; Dental pulp disease; Oral cancer.

Outline:
  1. Introduction
  2. PCR and RT-PCR description
    1. Polymerase chain reaction (PCR)
    2. Reverse transcription - polymerase chain reaction (RT-PCR)
  3. Literature review
    1. Periodontal disease
    2. Dental caries
    3. Endodontic infections
    4. Oral cancer
  4. Discussion
  5. Conclusions
  6. Test
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Release date: March 20, 2020
Review date: January 1, 2020
Expiration date: March 20, 2023
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